91572 Proteomics8cp; 11 x 2hpw, workshop; 1 x 20hpw, pratical
There are course requisites for this subject. See access conditions.
Anti-requisite(s): 91536 Proteomics
Proteomics describes the study of the complete set of proteins (proteome) that is expressed at a given time in a cell, tissue, organ or organism. Modern proteomics involves the integration of a wide range of protein analysis tools and information technologies to reliably identify qualitative and quantitative changes in proteins and their abundance; for example, in the detection of altered protein isoform (proteoform) abundance associated with disease. This subject covers leading technologies for sample preparation, protein fractionation, gel-based and chromatography-based separation and mass spectrometry for protein identification, characterisation and quantification.
Students explore facets of experimental design and data analysis through self-directed learning tasks and facilitated workshop activities. They are introduced to the best practice sample preparation technologies for different sample types, such as micro-organisms, plants, mammalian tissue and fluids. Written and oral assessment tasks require students to undertake independent research of relevant literature. The practical component allows students, through practice-related learning, to understand the logic and methodology underpinning sample preparation, complex mixture fractionation, protein separations and mass spectrometry. Students demonstrate their practice-related learning through the completion of a small laboratory-based project which requires a publication-style report encompassing data analysis skills.
Detailed subject description.