91567 Advanced Microscopy and Imaging8cp; 4 x 3hrs (practical), 1 x 3hrs (drop-in practical), 7 x 3hrs (computer analysis session), 2 x 1hr (tutorial) Distance students to negotiate alternative to on-campus learning with subject coordinator
There are course requisites for this subject. See access conditions.
Anti-requisite(s): 91566 Advanced Microscopy and Imaging
This subject provides an in-depth understanding of the theoretical principles of modern microscopy and offers students the opportunity for hands-on use of state-of-the-art research microscopes at UTS. The subject covers theoretical principles of light and fluorescence. Microscopy techniques include bright-field DIC, phase contrast and and dark-field microscopy. More advanced techniques based on fluorescence labelling fluorescence included widefield fluorescence with deconvolution and confocal microscopy. The subject reviews approaches to high resolution images and imformative live cell (real-time) imaging. The subject covers background and theory of some of the most recent and cutting-edge techniques including super resolution microscopy, FRET and FRAP. The subject reviews all aspects of sample preparation including fixation and mounting techniques. Optimised data acquisition for image analysis pipelines will be considered. The basic principles of image analysis for microscopy will be covered. Practical sessions provide the opportunity to perform both a fixed and a live cell fluorescent microscopy experiment and allow students to work with their own results data for complex computer analysis and presentation. Workshops enable students to design their own fluorescence microscopy experiment. A pilot experiment will inform the student designed major experiment. Results data from the major experiment in the subject are compiled for data analysis, presentation and discussion for a major written report presented in a fashion deemed acceptable in a peer-reviewed scientific journal.
Detailed subject description.