University of Technology Sydney

91566 Advanced Microscopy and Imaging

6cp; 8 x 1hr (lecture), 4 x 3hrs (practical), 1 x 3hrs (drop-in practical), 8 x 3hrs (computer analysis session), 4 x 2hrs (tutorial), 2 x 3hrs (planning workshop)
There are course requisites for this subject. See access conditions.
Anti-requisite(s): 91535 Microscopy and Cytometry


This subject provides an in-depth understanding of the theoretical principles of modern microscopy and offers students the opportunity for hands-on use of state-of-the-art research microscopes at UTS. The subject covers theoretical principles of light microscopy (bright-field and dark-field), fluorescence and epifluorescence, as well as deconvolution and confocal microscopy, and reviews issues in data-handling techniques for achieving high resolution and live cell (real-time) imaging. The subject covers background and theory of some of the most recent and cutting-edge techniques including super resolution microscopy, FRET and FRAP. The subject reviews all aspects of sample preparation, safety and staining techniques, as well as data-handling and storage, and extensive data analysis for microscopy. Practical sessions provide the opportunity to perform both a fixed and a live cell fluorescent microscopy experiment and allow students to work with their own results data for complex computer analysis and presentation. Workshops enable students to design their own fluorescence microscopy experiment. Results data in the subject are compiled for data analysis, presentation and discussion for a poster presentation and major written report presented in a fashion deemed acceptable in a peer-reviewed scientific journal.

Detailed subject description.

Access conditions

Note: The requisite information presented in this subject description covers only academic requisites. Full details of all enforced rules, covering both academic and admission requisites, are available at access conditions and My Student Admin.