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91565 Experimental and Diagnostic Flow Cytometry

6cp; 5hpw (most weeks): 1hr (lecture), 1hr (tutorial/workshop), 5hrs (laboratory) or 3hrs (data analysis class – there are two classes in weeks containing practical classes: a 3hr class followed by a 2hr class the next day)
There are course requisites for this subject. See access conditions.
Anti-requisite(s): 91535 Microscopy and Cytometry

Requisite elaboration/waiver:

Enrolment in a Master of Science coursework degree or a postgraduate course is required for entry into this subject. Bachelor of Advanced Science students may be permitted to enrol in this subject pending space availability and the approval of the subject coordinator.


This subject provides an in-depth understanding of the theoretical principles of contemporary multi-laser flow cytometry and offers students the opportunity for hands-on use of analytical flow cytometers at UTS. The subject covers the theoretical uses of light in flow cytometry including laser-excited fluorescence; band-pass filters and mirrors; diodes and photomultiplier tube (PMT) for photon amplification and detection; and the fluidics and each of the instrument components within a multi-colour analytical flow cytometer. Interactive lectures and tutorial sessions also review quality control, spectral analysis and cell sorting by flow cytometry. The essential elements of imaging cytometry and mass cytometry are covered. The subject also covers all aspects of sample preparation, flow cytometry, safety and cell staining techniques.

Practical class sessions provide an opportunity for students to generate their own flow cytometry data which gets analysed with industry-standard specialised flow cytometry software in weekly computer data analysis classes. Having learnt the basics of flow cytometry, students are then given the opportunity to design and perform their own unique flow cytometry experiment. Students analyse their personal experimental data individually and learn how to generate a journal-style report. Reports that are graded as distinction or above are eligible to be published in the annual student-based Journal of Microscopy and Cytometry Experiments (with the student's permission).

Detailed subject description.

Access conditions

Note: The requisite information presented in this subject description covers only academic requisites. Full details of all enforced rules, covering both academic and admission requisites, are available at access conditions and My Student Admin.